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The blackberry seed was typically removed as a byproduct and waste from blackberry fruits for juices. Developing value-added utilization of berry seeds will significantly expand the market for berry products as well as improve benefit to berry producers. However, the research on blackberry seed is limited.

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The objective of this paper was to research antithrombotic mechanism of polysaccharides in blackberry seeds. Polysaccharides in blackberry seeds were extracted, purified and identified by high-performance gel permeation chromatography (HPSEC), gas chromatography (GC), fourier transform infrared (FT-IR) spectrometer and nuclear magnetic resonance spectra (NMR). Anticoagulant activities were evaluated in vivo by measuring activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), fibrinogen (FIB) and plasma recalcification time (RRT).

Four polysaccharides named BSP-1a, BSP-1b, BSP-2 and BSP-3 were isolated from Blackberry ( Rubus spp.) seeds. The results indicated that BSP-1b, BSP-2 and BSP-3 exhibited the anticoagulant activity. Therefore, the anti-thrombosis effects of BSP-1b, BSP-2 and BSP-3 were investigated in vivo by 6-Keto-PGF 1α, thromboxane B 2 (TXB 2), endothelial nitric oxide synthase (eNOS), endothelin-1 (ET-1), whole blood viscosity (WBV), plasma viscosity (PV), hematocrit (Hct), erythrocyte sedimentation rate (ESR), APTT, TT, PT and FIB. The results suggested that BSP-1b, BSP-2 and BSP-3 had the inhibition effect on thrombus formation, and the antithrombotic effects were associated with the regulation of vascular endothelium active substance, activating blood flow and anticoagulation effect. Introduction Blackberry ( Rubus spp.) is a widely distributed shrub with high commercial value due to its sensory and chemical characteristics,such as sugars, malic acid, amino acids, VC, VE, anthocyanins, superoxide dismutase (SOD) and flavonoids [].

Therefore, it is usually used as raw material for the production of jams, candy, wine and juice beverages, and so on. The seeds of the blackberry fruits were typically removed during juice processing. In our previous research, 13 compounds were isolated and identified from blackberry seeds, pharmacology research indicated that blackberry seeds had antioxidant [], cholesterol-lowering [], liver-protection effects [], and could rectify the derangement in lipide metabolism []. However, there is no research on polysaccharides from blackberry seeds in literature. Plant polysaccharides display a wide variety of biological activity, including immunostimulatory [], anti-cancer [], anti-diabetic [] and antithrombotic activities [].